Journal: Cell reports

Article Title: Modeling Progressive Fibrosis with Pluripotent Stem Cells Identifies an Anti-fibrotic Small Molecule

doi: 10.1016/j.celrep.2019.11.019

Figure Lengend Snippet:

Article Snippet: Rabbit Vimentin (VIM) , Bioss , Cat# bs-0756R; RRID:AB_10855343.

Techniques: Recombinant, Luciferase, Imaging, Enzyme-linked Immunosorbent Assay, Hydroxyproline Assay, Software

Journal: Heliyon

Article Title: Characterization of primary human leptomeningeal cells in 2D culture

doi: 10.1016/j.heliyon.2024.e26744

Figure Lengend Snippet: Characterisation of the primary human leptomeningeal cells. Leptomeningeal cells stained for vimentin, cytokeratin and desmoplakin (green) and DAPI (blue). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: LMCs were stained for Vimentin [MAB2105, Biotechne], pan-Cytokeratin [AB7753, Abcam], Desmoplakin I + II [sc-390975, Santa-Cruz], CRABP2 [NB19051-7h12, Biotechne], S100A6 [NB100-2590, Biotechne], E-cadherin [SAB4503751, Sigma], Connexin-43 [AB11370, Abcam] and Occludin [SAB3500301, Sigma].

Techniques: Staining

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Cyclin A2, a novel regulator of EMT

doi: 10.1007/s00018-014-1654-8

Figure Lengend Snippet: Induction of mesenchymal traits following Cyclin A2 depletion in NMuMG cells. a Visualization of sh Luc and sh CycA2 NMuMG cells by light microscopy. Scale bar 200 μm. b Western blot analysis of E-Cadherin in Cyclin A2-depleted cells and sh Luc NMuMG cells. E-Cadherin expression was quantified by densitometry and normalized to that of GAPDH (n = 3, *P = 0.01). c Staining of F-Actin using Phalloidin-Rhodamine, β Catenin (red) or Vinculin (green), E-Cadherin (red) and p120 Catenin (green) in sh Luc and sh CycA2 NMuMG cells. Scale bar 20μm. d qPCR analysis of N-Cadherin, Fibronectin and MMP-14 mRNA levels. The values are expressed as fold change, where sh CycA2 was compared to sh Luc and TGFβ treated cells to untreated cells. Data are represented as mean ± SEM

Article Snippet: Antibodies used for immunofluorescence staining were: anti-Vinculin, (clone hVIN, Sigma), anti-E-Cadherin (clone36, BD Transduction Laboratory), anti-p120 Catenin (clone98, BD Transduction Laboratory), anti-β Catenin (clone 6B3, Cell Signaling), and anti-Vimentin (3932, Cell Signaling).

Techniques: Light Microscopy, Western Blot, Expressing, Staining

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Cyclin A2, a novel regulator of EMT

doi: 10.1007/s00018-014-1654-8

Figure Lengend Snippet: Impact of RhoA and RhoC knockdown ± that of Cyclin A2 in NMuMGRasV12 cells. a Invasion in collagen matrix (3D) of NMuMGRasV12 cells knocked down for both RhoC and Cyclin A2 (left panel) or for RhoA alone (right panel) (n = 3, ****P < 0.0001 for siRhoC and n = 3, ***P < 0.0002 for siRhoA). b Western blot analysis of E-Cadherin and RhoA depletion in NMuMGRasV12 cells. c, Analysis of E-Cadherin and p120 Catenin cellular localization in NMuMG and NMuMGRasV12 cells treated with siRNA RhoA. qPCR analysis of mRNA levels of EMT markers in NMuMGRasV12 cells knocked-down for RhoA (d) or both RhoC and Cyclin A2 (e). Results are shown as fold change of siRNA of interest knockdowns over control knockdowns (NMuMGRasV12 cells were used for the siRhoA and sh CycA2NMuMUGRasV12 cells for siRhoC). Data are represented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

Article Snippet: Antibodies used for immunofluorescence staining were: anti-Vinculin, (clone hVIN, Sigma), anti-E-Cadherin (clone36, BD Transduction Laboratory), anti-p120 Catenin (clone98, BD Transduction Laboratory), anti-β Catenin (clone 6B3, Cell Signaling), and anti-Vimentin (3932, Cell Signaling).

Techniques: Knockdown, Western Blot, Control

Journal: Cell reports

Article Title: PCDH12 loss results in premature neuronal differentiation and impeded migration in a cortical organoid model

doi: 10.1016/j.celrep.2023.112845

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Phospho-Vimentin (Ser56) polyclonal antibody , Cell Signaling Technology , Cat #: 3877, RRID: AB_2216265.

Techniques: Purification, Recombinant, Membrane, TUNEL Assay, Protein Extraction, Clinical Proteomics, Isolation, Cell Fractionation, In Vivo, Bicinchoninic Acid Protein Assay, Sequencing, Introduce, Variant Assay, Plasmid Preparation, Software, Chemotaxis Assay, Migration

Journal: Scientific Reports

Article Title: TMBIM1 promotes epithelial mesenchymal transition by accelerating autophagic degradation of E-cadherin in glioblastoma

doi: 10.1038/s41598-025-01699-4

Figure Lengend Snippet: Inhibition of autophagy reverses EMT in GBM cells. ( A, C ) Inhibition of autophagy by CQ and 3-MA reduced the migration and invasion of U251 cells with TMBIM1 knockdown/overexpression. ( B, D ) Statistical analysis of cells per field in ( A ) and ( C ). ( E ) Inhibition of autophagy reversed the changes in the expression of epithelial markers and mesenchymal markers in TMBIM1-overexpressing cells and further increased E-cadherin expression and decreased N-cadherin, vimentin and SNAI1 expression in TMBIM1-knockdown cells. One-way analysis of variance (ANOVA) was used measure statistical significance, and Tukey’s test was used to compare differences between groups. * P < 0.05, ** P < 0.01, ***P < 0.001.

Article Snippet: Antibodies specific for E-cadherin (20874-1-AP), N-cadherin (22018-1-AP), Vimentin (10366–1-AP), SNAI1 (13099-1-AP), P62 (18420–1-AP), Beclin1 (11306-1-AP), AMPK (66536-1-Ig) and ULK1 (20986-1-AP) were obtained from Proteintech (Wuhan, China).

Techniques: Inhibition, Migration, Knockdown, Over Expression, Expressing

Journal: Scientific Reports

Article Title: TMBIM1 promotes epithelial mesenchymal transition by accelerating autophagic degradation of E-cadherin in glioblastoma

doi: 10.1038/s41598-025-01699-4

Figure Lengend Snippet: TMBIM1 knockdown suppresses autophagy and EMT in an intracranial xenograft model. ( A ) In vivo bioluminescence imaging of nude mice 30 days after cell inoculation. ( B ) Mouse survival was shown by Kaplan‒Meier analysis. The log-rank test was used to measure survival differences. ( C ) HE staining. ( D ) IHC staining of p-AMPK (Thr172), p-ULK1 (Ser317), p-mTOR (Ser2448), Beclin1, P62 and TMBIM1. Scale bars, 20 um. ( E ) IHC staining of E-cadherin, N-cadherin, Vimentin and SNAI1. Scale bars, 20 um.

Article Snippet: Antibodies specific for E-cadherin (20874-1-AP), N-cadherin (22018-1-AP), Vimentin (10366–1-AP), SNAI1 (13099-1-AP), P62 (18420–1-AP), Beclin1 (11306-1-AP), AMPK (66536-1-Ig) and ULK1 (20986-1-AP) were obtained from Proteintech (Wuhan, China).

Techniques: Knockdown, In Vivo, Imaging, Staining, Immunohistochemistry